Vol. 7, Issue 2, Part B (2025)

Murraya koenigii (curry leaves) are a great source of valuable nutrients and bioactive compounds. Using Fourier Transform Infrared Spectroscopy, a quick and non-destructive analytical method, the current study sought to assess the phyto-constituents of curry leaves. Ethanol leaf extract was produced using a Soxhlet extraction method. Thin-layer chromatography was performed on the ethanolic leaf extract of Murraya koenigii to evaluate the solvent systems for separating the secondary metabolites. The mobile phases tested were n-propanol: formic acid: water (20:3:2), ethyl acetate: n-hexane (15:10), and glacial acetic acid: water: n-butanol (5:5:15), which were further applied for column chromatography. The Soxhlet-derived extract is subjected to column fractionation, and the collected fractions are used to identify functional groups in plant extracts by using Fourier Transform Infrared spectroscopy. From this study, it can be concluded that the extract consisted of various functional groups, including ether, alcohol, amine, alkyl halide, and aromatic ring, with characteristic absorption bands observed at 1078 cm^⁻¹, 3458 cm^⁻¹, 1592 cm^⁻¹, 660-564 cm^⁻¹, and 1567 cm^⁻¹ wavelengths, respectively, in the FTIR spectrum. The study will be explored for integration with other analytical techniques and quality applications, with prospects in advanced research, clinical validation, and herbal formulation.