Vol. 7, Issue 2, Part B (2025)

Artocarpus altilis (A. altilis), commonly known as breadfruit, is widely exploited for food and non-food uses. In Southeast Asian regions, the leaves of A. altilis are consumed for their medicinal properties following traditional medicine. A growing body of literature points toward the extract of A. altilis leaves possessing anti-cancer, antioxidative, photoprotective, anti-inflammatory, hepatoprotective, renoprotective, and cardioprotective properties. Furthermore, experimental and computational methods have demonstrated that there is an association between the antioxidative property with the flavonoid and phenolic contents of A. altilis extract. In an attempt to identify the optimal extraction parameters for the Soxhlet extraction of A. altilis leaves, the extraction time and the sample-to-solvent ratio were optimized by one-factor-at-a-time methodology. The optimized extraction parameters were identified as: 6-hour Soxhlet extraction, sample-to-solvent ratio of 4:160 (g:mL), with ethanol:water (60:40, v/v) as solvent. The optimized A. altilis leaf extract exhibited total phenolic content and total flavonoid content values of 86.96±0.2130 mg GAE g⁻¹ DW and 33.76±0.2991 mg QE g⁻¹ DW, respectively. The optimized A. altilis leaf extract demonstrated potential for the development of nutraceutical and pharmaceutical products due to its strong antioxidant activity.