Vol. 6, Issue 1, Part A (2024)

Manilkara zapota leaves extracts were investigated for its bioactive compounds by screening phytochemically and quantitatively, along with characterization of the biocompounds usingaqueous, petroleum ether, ethyl acetate, chloroform and ethanol extracts. Qualitative analysis revealed the presence of phenolic groups, flavonoids and triterpenoids in the ethanol and aqueous extract of the fruit. Total phenol content of the fruit in the aqueous and ethanol extract was 27.8 and 90.1 mg/100 gm, Flavonoid content was 39.3 and 106 mg/100 gm and Total triterpenoid was 49.9 and 61.9 mg/100 gm respectively. Antioxidant potential of M. zapota leaves extracts estimated through the DPPH radical scavenging activity and superoxide anion scavenging activity showed variation in the reduction potential. Antioxidant activity of the extracts estimated through the DPPH radical scavenging activity at different concentrations of 20, 40, 60, 80 and 100 μg /ml showed higher reduction ability at increasing concentration. The IC50 value through DPPH assay of the ethanol extract (86.2 μg /ml) followed by aqueous, ethyl acetate, chloroform and petroleum ether extract. Superoxide anion radical scavenging activity of M. zapota leaves extracts showed higher scavenging activity in methanolic extract. An IC50value of 74.62 μg /ml was shown by ethanolic fruit extract through Superoxide anion radical scavenging assay. The GC-MS analysis of methanolic leavesextract revealed the presence of 25 compounds which were eluted at various intervals of time. The compound 2-(Acetoxymethyl)-3-(Methoxycarbonyl) biphenylene (21st compound) showed the highest sharp peak 27.19 with the retention time (RT) of 16.516 minutes. Retention time (RT) of 8.526 minutes denoted the peak 0.22 which corresponded to the compound identified as Bicyclo (4.3.0) nonane, 3-methylene-.The work is discussed in detail.